MBP tag Monoclonal antibody

MBP tag Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

4C6H4

Cat no : 66003-1-Ig

Synonyms

Maltose Binding Protein, MBP, MBP Tag


"MBP tag Antibodies" Comparison

View side-by-side comparison of MBP tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • Western Blot (WB) analysis of Recombinant protein using MBP tag Monoclonal antibody (66003-1-Ig)

    WB analysis of Recombinant protein using 66003-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Recombinant protein using MBP tag Monoclonal antibody (66003-1-Ig)

    IP experiment of Recombinant protein using 66003-1-Ig

    IP Result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected cells using MBP tag Monoclonal antibody (66003-1-Ig)

    IF Staining of Transfected cells using 66003-1-Ig

    IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University.


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inRecombinant protein protein
Positive IF detected inTransfected cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66003-1-Ig targets MBP tag in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MBP tag fusion protein Ag0942
Full Name MBP tag
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Protocols

Product Specific Protocols
WB protocol for MBP tag antibody 66003-1-IgDownload protocol
IP protocol for MBP tag antibody 66003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination.

Authors - Yansong Zhang
WB

Circ Res

Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction.

Authors - Guizhen Zhao
WB

Nat Commun

Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet.

Authors - Song Liu
WB,IP

Nat Commun

The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.

Authors - Ying Zhang
IF

EMBO J

Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation.

Authors - Lara A Gruijs da Silva

Plant Cell

Activation and negative feedback regulation of SlHY5 transcription by the SlBBX20/21-SlHY5 transcription factor module in UV-B signaling.

Authors - Guoqian Yang