HRP-conjugated GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum

Applications

WB

Conjugate

HRP Fluorescent Dye

Cat no : HRP-66001

Synonyms

GST, GST tag



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 10 publications below

Product Information

HRP-66001 targets GST Tag in WB applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

Tested Reactivity recombinant protein, schistosoma japonicum
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST Tag
Calculated molecular weight 26 kDa
Observed molecular weight-
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

SpeciesApplicationTitle
WB

J Immunol Res

TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer.

Authors - BiKang Yang
WB

Nat Metab

The SESAME complex regulates cell senescence through the generation of acetyl-CoA.

Authors - Wanping Chen
WB

Virus Genes

The host cellular protein Ndufaf4 interacts with the vesicular stomatitis virus M protein and affects viral propagation.

Authors - Wei Pan

Vet Microbiol

The matrix protein of vesicular stomatitis virus inhibits host-directed transcription of target genes via interaction with the TFIIH subunit p8.

Authors - Wei Pan
WB

Vet Microbiol

Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein.

Authors - Yanlong Zhou
WB

Vet Microbiol

EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein.

Authors - Wei Pan