A4GALT Polyclonal antibody

A4GALT Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12392-1-AP

Synonyms

A4GALT, A4GALT1, CD77 synthase, Gb3 synthase



Tested Applications

Positive WB detected inRAW 264.7 cells, K-562 cells, Raji cells
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12392-1-AP targets A4GALT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen A4GALT fusion protein Ag3069
Full Name alpha 1,4-galactosyltransferase
Calculated molecular weight 353 aa, 41 kDa
Observed molecular weight 50-52 kDa
GenBank accession numberBC017068
Gene symbol A4GALT
Gene ID (NCBI) 53947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for A4GALT antibody 12392-1-APDownload protocol
IHC protocol for A4GALT antibody 12392-1-APDownload protocol
IF protocol for A4GALT antibody 12392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols