Tyramide Signaling Amplification (TSA) Reagents
Achieve up to 5-plex imaging with our CoraLite® Plus-Tyramide Reagents and Detection Kits
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Up to 100x more sensitive than indirect staining methods
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Achieve publication quality data at the first attempt
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Wide Dynamic Range enabling the detection of low abundance targets
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Easy multiplexing with no limitations on primary antibody host
Proteintech’s CoraLite® Plus-Tyramide dyes are fluorescent labeling reagents based on TSA technology. Upon interaction with HRP, the tyramides are oxidized into free radicals which then allows the dyes to form covalent bonds with nearby tyrosine residues on the antigen of interest.
Compared to indirect staining methods, TSA dyes can enhance the fluorescent signal by several hundred-fold due to dye accumulation and direct labeling of the target. This makes them particularly useful for the detection of low abundance targets.
Furthermore, this system is highly-suitable for tissue multiplexing as multiple TSA dyes can be sequentially added through cyclical antibody stripping and re-probing.
Increased Sensitivity, Ideal for Low Abundance Targets
Detection of IL-17RA Polyclonal Antibody (32055-1-AP) in A431 Cells
With Multi-rAb CoraLite Plus 594 Recombinant Secondary (RGAR004)
With Multi-rAb Polymer-HRP Recombinant Secondary (RGAR011) and CoraLite® Plus 594 Tyramide Reagent (PR30024)
Achieve High-Quality Multiplexed-Data
FFPE-rat brain tissue was stained with Lamin B1 monoclonal antibody (Cat.NO. 66095-1-Ig) at 1:2000 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLite®Plus 555-Tyramide Reagent was used for signal development (yellow). HIER was then performed in pH 9.0 Tris-EDTA buffer followed by staining with GFAP monoclonal antibody (60190-1-Ig, 1:20000). CoraLite®Plus 488-Tyramide Reagent were used for signal development (green). Similarly, IBA1 recombinant antibody (81728-1-RR, 1:2000) TDP-43 recombinant antibody (80002-1-RR, 1:3200) were stained subsequently. CoraLite®Plus 594-Tyramide Reagent (red) and CoraLite®Plus 647-Tyramide Reagent (purple) were used for signal development, respectively. Finally, cell nucleus (blue) were stained with DAPI.
FFPE-human tonsillitis tissue was stained with CD23 monoclonal antibody (60208-2-Ig) at 1:8000 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLite®Plus 555-Tyramide Reagent was used for signal development (yellow). HIER was then performed in pH 9.0 Tris-EDTA buffer followed by staining with CD3 monoclonal antibody (60181-1-Ig, 1:10000). CoraLite®Plus 488-Tyramide Reagent were used for signal development (green). Similarly, CD8a monoclonal antibody (66868-1-Ig, 1:20000) and CD138 monoclonal antibody (67155-1-Ig, 1:10000) were stained subsequently. CoraLite®Plus 594-Tyramide Reagent (red) and CoraLite®Plus 647-Tyramide Reagent (purple) were used for signal development respectively. Finally, cell nucleus (blue) were stained with DAPI.
Our Offering
CoraLite® Plus- Tyramide Reagents
CoraLite® Plus-Tyramide Reagents can be used with any HRP-conjugated secondary antibody for multiplexed IHC, ICC, or ISH experiments
| Dye | Catalog No. |
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| CoraLite® Plus 488-Tyramide Reagent | PR30022 |
| CoraLite® Plus 555-Tyramide Reagent | PR30023 |
| CoraLite® Plus 594-Tyramide Reagent | PR30024 |
| CoraLite® Plus 647-Tyramide Reagent | PR30025 |
TSA Detect Kits
TSA Detect Kits are a complete fluorescent IHC workflow solution for tissue multiplexing. They include either 3 or 4 tyramide reagents, DAPI nuclear stain, a Multi-rAb Poly HRP Recombinant Secondary, quenching buffer, blocking buffer, and amplification buffer.