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ABCD4 Polyclonal antibody

ABCD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15935-1-AP

Synonyms

ABC41, ABCD4, EST352188, P70R, P79R, PMP69, PMP70 related protein, PXMP1 L, PXMP1L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCD4 Polyclonal antibody (15935-1-AP)

    WB analysis using 15935-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15935-1-AP (ABCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15935-1-AP targets ABCD4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCD4 fusion protein Ag8783
Full Name ATP-binding cassette, sub-family D (ALD), member 4
Calculated Molecular Weight 606 aa, 69 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC012815
Gene Symbol ABCD4
Gene ID (NCBI) 5826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCD4, also known as P70R, PMP69, and PXMP1L, belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCD4 Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner (PMID: 22922874, 33845046, 28572511). ABCD4 is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism, which is related to methylmalonic aciduria and homocystinuria type cblJ (MAHCJ).

Protocols

Product Specific Protocols
WB protocol for ABCD4 antibody 15935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols