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  • Featured Product
  • KD/KO Validated

ACAP3 Polyclonal antibody

ACAP3 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17570-1-AP

Synonyms

ACAP3, Centaurin beta 5, CENTB5, Cnt b5, KIAA1716

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ACAP3 Polyclonal antibody (17570-1-AP)

    WB analysis of Jurkat using 17570-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ACAP3 Polyclonal antibody (17570-1-AP)

    WB analysis of SH-SY5Y using 17570-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ACAP3 Polyclonal antibody (17570-1-AP)

    IF Staining of SH-SY5Y using 17570-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, SH-SY5Y cells
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17570-1-AP targets ACAP3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAP3 fusion protein Ag11777
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
Calculated Molecular Weight 834 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC047001
Gene Symbol ACAP3
Gene ID (NCBI) 116983
RRIDAB_2257668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACAP3 antibody 17570-1-APDownload protocol
IF protocol for ACAP3 antibody 17570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Biochem J

ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons.

Authors - Yuki Miura
View Article
mouseWB

Biochem Biophys Res Commun

ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.

Authors - Yuki Miura
  • KD Validated
View Article