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ASIC2 Polyclonal antibody

ASIC2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 17851-1-AP

Synonyms

ACCN, ACCN1, Acid sensing ion channel 2, ASIC2, ASIC2a, BNaC1, BNC1, Brain sodium channel 1, hBNaC1, Mammalian degenerin homolog, MDEG

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using ASIC2 Polyclonal antibody (17851-1-AP)

    WB analysis of rat brain using 17851-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17851-1-AP (ASIC2 antibody at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ASIC2 Polyclonal antibody (17851-1-AP)

    IP experiment of mouse brain using 17851-1-AP

    IP result of anti-ASIC2 (IP:17851-1-AP, 4ug; Detection:17851-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Tested Applications

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 17851-1-AP is ASIC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASIC2 fusion protein Ag12301
Full Name amiloride-sensitive cation channel 1, neuronal
Calculated Molecular Weight 512 aa, 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC075043
Gene Symbol ACCN1
Gene ID (NCBI) 40
RRIDAB_2878451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASIC2 (acid-sensing ion channel 2), also known as ACCN1 (amiloride-sensitive cation channel 1, neuronal), is a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. ASIC2 is widely expressed in the central nervous system (CNS), may playing a role in neurotransmission. It has two alternatively spliced variants, ASIC2a and ASIC2b.

Protocols

Product Specific Protocols
WB protocol for ASIC2 antibody 17851-1-APDownload protocol
IP protocol for ASIC2 antibody 17851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.

Authors - Jingyu Ding
View Article
humanWB

Sci Rep

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons.

Authors - Junyu Xu
View Article