beta actin Monoclonal antibody

beta actin Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7A9H3

Cat no : 60008-2-Ig

Synonyms



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

60008-2-Ig targets beta actin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen beta actin fusion protein Ag0297
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC002409
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic Acid/Ammonium Sulfate Precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.

Publications

SpeciesApplicationTitle
mouseWB

Cell Cycle

Negative impact of hyperglycaemia on mouse alveolar development.

Authors - Mei-Yao He

Neurochem Res

β-Caryophyllene Attenuates Focal Cerebral Ischemia-Reperfusion Injury by Nrf2/HO-1 Pathway in Rats.

Authors - Jie Lou
mouseWB

Brain Res Bull

GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose.

Authors - Bin Xu
humanWB

Amino Acids

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Authors - Chen Bai

J Exp Clin Cancer Res

Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation.

Authors - Su Yao
ratWB

Food Chem Toxicol

3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.

Authors - Jing Lu