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beta actin Antibody 5 Publications

Mouse Monoclonal| Catalog number: 60008-2-Ig |CloneNo.: 7A9H3

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, ELISA
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Species specificity:
human, mouse
Recommended dilution:

Protocols

Product-specific protocols

    Standard Protocols

    Product Information

    Source:
    Mouse
    Purification method:
    Caprylic Acid/Ammonium Sulfate Precipitation
    Isotype:
    IgM
    Storage:
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

    Immunogen Information

    Immunogen:
    beta actin fusion protein Ag0297
    Full name:
    actin, beta
    Calculated molecular weight:
    375aa,42 kDa
    Observed molecular weight:
    45 kDa
    GenBank accession number:
    BC002409
    Gene ID (NCBI):
    60
    Gene symbol
    ACTB
    Synonyms

    Background

    Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.


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