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beta actin Antibody 5 Publications

Mouse Monoclonal| Catalog number: 60008-2-Ig |CloneNo.: 7A9H3

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, ELISA
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Species specificity:
human, mouse
Recommended dilution:
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Caprylic Acid/Ammonium Sulfate Precipitation
Isotype:
IgM
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
beta actin fusion protein Ag0297
Full name:
actin, beta
Calculated molecular weight:
375aa,42 kDa
Observed molecular weight:
45 kDa
GenBank accession number:
BC002409
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms

Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.


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