Beta Actin Monoclonal antibody

beta actin Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (3)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

7A9H3

Cat no : 60008-2-Ig

Synonyms



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60008-2-Ig targets beta actin in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen beta actin fusion protein Ag0297
Full Name actin, beta
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC002409
Gene Symbol ACTB
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic Acid/Ammonium Sulfate Precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.

Publications

SpeciesApplicationTitle
humanIP

Nat Commun

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.

Authors - Xinzhi Li
mouseWB

Cell Mol Immunol

MEF2C promotes M1 macrophage polarization and Th1 responses.

Authors - Xibao Zhao
humanWB

Elife

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress

Authors - Jinyang Wang
humanIP

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang
ratWB

Bioeng Transl Med

Neonatal microglia and proteinase inhibitors-treated adult microglia improve traumatic brain injury in rats by resolving the neuroinflammation.

Authors - Ping Zheng
mouseWB

PLoS Genet

Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing.

Authors - Sherylanne Newton