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  • KD/KO Validated

ADAM28 Polyclonal antibody

ADAM28 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22234-1-AP

Synonyms

ADAM 28, ADAM28, eMDC II, eMDCII, MDC L, MDC Lm, MDC Ls, MDCL



Tested Applications

Positive WB detected inmouse lung tissue, BxPC-3 cells, mouse colon tissue, mouse liver tissue, mouse small intestine tissue, mouse spleen tissue, PC-13 cells
Positive IP detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22234-1-AP is ADAM28 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM28 fusion protein Ag17586
Full Name ADAM metallopeptidase domain 28
Calculated molecular weight 775 aa, 87 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC136478
Gene symbol ADAM28
Gene ID (NCBI) 10863
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).

Protocols

Product Specific Protocols
WB protocol for ADAM28 antibody 22234-1-APDownload protocol
IP protocol for ADAM28 antibody 22234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Neurosci

Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.

Authors - Zhen-Huang Chen
humanWB

World J Gastroenterol

PMID: 31602160

Authors - Li Wei
  • KD Validated
humanWB

Cancer Lett

ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.

Authors - Jia-Min Zhang
  • KO Validated