Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11232-2-AP targets AEBP2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1756 Product name: Recombinant human AEBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-295 aa of BC015624 Sequence: MSSDGEPLSRMDSEDSISSTIMDVDSTISSGRSTPAMMNGQGSTTSSSKNIAYNCCWDQCQACFNSSPDLADHIRSIHVDGQRGGVFVCLWKGCKVYNTPSTSQSWLQRHMLTHSGDKPFKCVVGGCNASFASQGGLARHVPTHFSQQNSSKVSSQPKAKEESPSKAGMNKRRKLKNKRRRSLPRPHDFFDAQTLDAIRHRAICFNLSAHIESLGKGHSVVFHSTVIAKRKEDSGKIKLLLHWMPEDILPDVWVNESERHQLKTKVVHLSKLPKDTALLLDPNIYRTMPQKRLKR Predict reactive species |
| Full Name | AE binding protein 2 |
| Calculated Molecular Weight | 33 kDa, 54 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC015624 |
| Gene Symbol | AEBP2 |
| Gene ID (NCBI) | 121536 |
| RRID | AB_2225962 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN18 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AEBP2, also named as Zinc finger protein AEBP2 or Adipocyte enhancer-binding protein 2, is a 517 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the AEBP2/jing C2H2-type zinc-finger family. AEBP2 as a DNA-binding transcriptional repressor may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. It may e playing a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for AEBP2 antibody 11232-2-AP | Download protocol |
| WB protocol for AEBP2 antibody 11232-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AEBP2 Polyclonal antibody (Cat# 11232-2-AP) has 5 total citations published in Nature, Molecular Cancer, Nucleic Acids Research, PLoS Genetics, and PLoS ONE. The associated research fields include epigenetic regulation, transcriptional regulation in embryonic stem cells, ovarian cancer and cisplatin resistance, neural crest cell development, and atherogenic gene network modulation.
| Species | Application | Title |
|---|---|---|
Nature Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells. | ||
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nucleic Acids Res AEBP2 as a potential targeting protein for Polycomb Repression Complex PRC2. | ||
PLoS Genet Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. | ||
