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AFM Polyclonal antibody

AFM Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 19494-1-AP

Synonyms

afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using AFM Polyclonal antibody (19494-1-AP)

    WB analysis of human plasma using 19494-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 19494-1-AP (AFM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AFM Polyclonal antibody (19494-1-AP)

    IF Staining of HepG2 using 19494-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 19494-1-AP (AFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19494-1-AP targets AFM in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name afamin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberNM_001133
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2878581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

Protocols

Product Specific Protocols
WB protocol for AFM antibody 19494-1-APDownload protocol
IF protocol for AFM antibody 19494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Afamin promotes glucose metabolism in papillary thyroid carcinoma.

Authors - Chen-Tian Shen
View Article