AIFM2/ FSP1 Polyclonal antibody

AIFM2/ FSP1 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20886-1-AP

Synonyms

AIFM2, AMID, Apoptosis inducing factor 2, ferroptosis suppressor protein 1, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2



Tested Applications

Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20886-1-AP is AIFM2/ FSP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag13412
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC023601
Gene symbol AIFM2
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Protocols

Product Specific Protocols
IHC protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
IP protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Int Immunopharmacol

Impact of Wortmannilactone F and G31P on Clonorchis Sinensis-infected mice.

Authors - Yuanhua Qin
humanWB

Am J Cancer Res

PMID: 32368394

Authors - Zeng Ye
humanWB

Nature

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.

Authors - Kirill Bersuker