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AIFM2 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 20886-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
IP, IHC, ELISA
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Images See all

AIFM2 Antibody IP L02 cells 20886-1-AP

IP Result of anti-AIFM2 (IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:300) with L02 cells lysate 1800ug.

AIFM2 Antibody IHC human liver tissue 20886-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 20886-1-AP( AIFM2 Antibody) at dilution of 1:200 (under 10x lens).

AIFM2 Antibody IHC human liver tissue 20886-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 20886-1-AP( AIFM2 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human
Positive IP detected in:
L02 cells
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AIFM2 fusion protein Ag13412
Full name:
apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight:
41 kDa
Observed molecular weight:
41 kDa
GenBank accession number:
BC023601
Gene ID (NCBI):
84883
Gene symbol
AIFM2
Synonyms
AIFM2, AMID, Apoptosis inducing factor 2, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

Background

The human AIFM2 protein (formerly known as AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.


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