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AKR7A2 Polyclonal antibody

AKR7A2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10730-1-AP

Synonyms

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase



Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, A431 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

10730-1-AP targets AKR7A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR7A2 fusion protein Ag1049
Full Name aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007352
Gene symbol AKR7A2
Gene ID (NCBI) 8574
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells (PMID:22001351).

Protocols

Product Specific Protocols
WB protocol for AKR7A2 antibody 10730-1-APDownload protocol
IHC protocol for AKR7A2 antibody 10730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Pancreatology

Proteomic analysis of pancreatic ductal adenocarcinoma compared with normal adjacent pancreatic tissue and pancreatic benign cystadenoma.

Authors - Cui Yazhou Y