ALDH1B1 Antibody

ALDH1B1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22220-1-AP

Synonyms

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The 22220-1-AP immunogen is Fusion Protein ALDH1B1 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1B1 fusion protein Ag17557
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated molecular weight 517aa,57 kDa
Observed molecular weight 55-58 kDa
GenBank accession number BC001619
Gene symbol ALDH1B1
Gene ID (NCBI) 219
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IF protocol for ALDH1B1 antibody 22220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oncotarget

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Authors - Hao-Long Zeng