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Angiopoietin 1 Polyclonal antibody

Angiopoietin 1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 23302-1-AP

Synonyms

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, ANGPT1, KIAA0003



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue, HepG2 cells, A375 cells, rat heart tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23302-1-AP is Angiopoietin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Angiopoietin 1 fusion protein Ag18829
Full Name angiopoietin 1
Calculated molecular weight 498 aa, 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC152411
Gene symbol ANGPT1
Gene ID (NCBI) 284
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein synthesized in pericytes.(PMID: 19786610). It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin 1 is fundamental to physiological angiogenesis, including endothelial cell survival, vascular branching, and pericyte recruitment. ANGPT1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of ANGPT1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Protocols

Product Specific Protocols
WB protocol for Angiopoietin 1 antibody 23302-1-APDownload protocol
IF protocol for Angiopoietin 1 antibody 23302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Biomed Pharmacother

Sinomenine mitigates collagen-induced arthritis mice by inhibiting angiogenesis.

Authors - Zhi-Tao Feng
ratWB

CNS Neurosci Ther

Dl-3-N-butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats.

Authors - Pan-Ting Zhou
humanWB

J Cell Mol Med

IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction.

Authors - Da Wo
ratWB

Neurosci Lett

Repulsive guidance molecule a suppresses angiogenesis after ischemia/reperfusion injury of middle cerebral artery occlusion in rats.

Authors - Yu Wang
humanWB

Int J Oncol

MicroRNA‑21 contributes to renal cell carcinoma cell invasiveness and angiogenesis via the PDCD4/c‑Jun (AP‑1) signalling pathway.

Authors - Bo Fan
humanWB,IHC

Angiogenesis

miR-153 inhibits the migration and the tube formation of endothelial cells by blocking the paracrine of angiopoietin 1 in breast cancer cells.

Authors - Huichun Liang
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