|Positive WB detected in||mouse heart tissue, mouse liver tissue, HepG2 cells, A375 cells, rat heart tissue|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23302-1-AP is Angiopoietin 1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Angiopoietin 1 fusion protein Ag18829|
|Full Name||angiopoietin 1|
|Calculated molecular weight||498 aa, 58 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC152411|
|Gene ID (NCBI)||284|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Angiopoietin 1 is a 70-kDa secreted glycoprotein synthesized in pericytes.(PMID: 19786610). It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin 1 is fundamental to physiological angiogenesis, including endothelial cell survival, vascular branching, and pericyte recruitment. ANGPT1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of ANGPT1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.
Sinomenine mitigates collagen-induced arthritis mice by inhibiting angiogenesis.
CNS Neurosci Ther
Dl-3-N-butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats.
J Cell Mol Med
IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction.
Repulsive guidance molecule a suppresses angiogenesis after ischemia/reperfusion injury of middle cerebral artery occlusion in rats.
Int J Oncol
MicroRNA‑21 contributes to renal cell carcinoma cell invasiveness and angiogenesis via the PDCD4/c‑Jun (AP‑1) signalling pathway.
miR-153 inhibits the migration and the tube formation of endothelial cells by blocking the paracrine of angiopoietin 1 in breast cancer cells.