Tested Applications
| Positive WB detected in | HUVEC cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A375 cells, rat heart tissue, HeLa cells, K-562 cells, C6 cells, U-87 MG cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 32 publications below |
| IF | See 4 publications below |
Product Information
23302-1-AP targets Angiopoietin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18829 Product name: Recombinant human ANGPT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 161-293 aa of BC152411 Sequence: IQLLENSLSTYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRATTNNSVLQKQQLELMDTVHNLVNLCTKEGVLLKGGKREEEKPFRDCADVYQAG Predict reactive species |
| Full Name | angiopoietin 1 |
| Calculated Molecular Weight | 498 aa, 58 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC152411 |
| Gene Symbol | Angiopoietin 1 |
| Gene ID (NCBI) | 284 |
| RRID | AB_2879250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15389 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
| WB protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomater Res High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration | ||
Acta Biomater Chemotactic Recruitment of Genetically Engineered Cell Membrane-Camouflaged Metal-Organic Framework Nanoparticles for Ischemic Osteonecrosis Treatment | ||
Mol Ther Nucleic Acids Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC. | ||
Neural Regen Res Endorepellin downregulation promotes angiogenesis after experimental traumatic brain injury | ||
J Cell Mol Med IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction. | ||
Br J Pharmacol Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis. |
