ANGPTL3 Polyclonal antibody

ANGPTL3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11964-1-AP

Synonyms

ANG 5, Angiopoietin 5, angiopoietin like 3, Angiopoietin like protein 3, Angiopoietin related protein 3, ANGPT5, ANGPTL3



Tested Applications

Positive WB detected inA375 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11964-1-AP targets ANGPTL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANGPTL3 fusion protein Ag2573
Full Name angiopoietin-like 3
Calculated Molecular Weight 460 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC058287
Gene Symbol ANGPTL3
Gene ID (NCBI) 27329
RRIDAB_874076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.

Protocols

Product Specific Protocols
WB protocol for ANGPTL3 antibody 11964-1-APDownload protocol
IHC protocol for ANGPTL3 antibody 11964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Invest Ophthalmol Vis Sci

PMID: 30128492

Authors - Na Wang
humanWB

Cancer Sci

Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray

Authors - Yongdong Yi