Beta Amyloid Monoclonal antibody

Beta Amyloid Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5C2A1

Cat no : 60342-1-Ig

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta



Tested Applications

Positive WB detected inY79 cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60342-1-Ig targets Beta Amyloid in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Beta Amyloid fusion protein Ag0769
Full Name amyloid beta (A4) precursor protein
Calculated molecular weight 87 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC004369
Gene symbol APP
Gene ID (NCBI) 351
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).

Protocols

Product Specific Protocols
WB protocol for Beta Amyloid antibody 60342-1-IgDownload protocol
IHC protocol for Beta Amyloid antibody 60342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cardiovasc Pathol

Sudden unexpected death in early Parkinson's disease: neurogenic or cardiac death?

Authors - Naoki Nishida
mouseWB,IF

Aging (Albany NY)

Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease.

Authors - Chao Zhou
humanWB

Cell Rep

CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.

Authors - Yang-Ping Shentu