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androgen receptor Antibody

androgen receptor Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 22089-1-AP

Synonyms

AIS, androgen receptor, AR, DHTR, Dihydrotestosterone receptor, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM



Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, LNCaP cells, NIH/3T3 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, mouse ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 22089-1-AP immunogen is Fusion Protein androgen receptor protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen androgen receptor fusion protein Ag17291
Full Name androgen receptor
Calculated molecular weight 914aa,99 kDa
Observed molecular weight 75-80 kDa,100-110 kDa
GenBank accession number BC132975
Gene symbol AR
Gene ID (NCBI) 367
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

1. What is the molecular weight of AR? Are there any isoforms of AR?

The molecular weight of full-length androgen receptor (AR-A) is 110 kDa. An additional variant, AR-B, has an 87 kDa size and lacks the N-terminal 187 amino acids of AR-A (PMID: 8108393). Recently, more splice variants of AR have been discovered, raising protein products of around 80 kDa length (PMID: 19244107), as well as an AR45 variant of 45 kDa size (PMID: 15634333). AR splice variants differ in their cell line-specific expression (PMID: 24570075).

2. What is the subcellular localization of AR?

AR can be present in either or both of the cytoplasm and nucleus. In androgen-deprived cells, AR is found predominantly in the cytoplasm, while stimulation by androgens causes enrichment of androgen-bound AR in the nucleus. AR shuttles between the cytoplasm and nucleus and its phosphorylation state has an impact on the subcellular localization (PMID: 16282370).

3. Is AR post-translationally modified?

Post-translational modifications of the AR include phosphorylation, acetylation, methylation, SUMOylation, and ubiquitination (PMID: 21820033). These modifications have an impact on receptor stability, activity, and can change the observed molecular weight of the AR.

4. How to study AR signaling in cell culture?

It is important to control levels of cell stimulation while also looking at AR signaling. Fetal bovine serum (FBS) that is typically used in cell culture contains low levels of testosterone that are enough to stimulate the growth of prostate cells (PMID: 19676093), including the LNCaP cell line that is a commonly used human prostatic carcinoma cell model (PMID: 6831420). One possibility for complete testosterone deprivation is to use charcoal stripped FBS that removes lipophilic agents, including androgens. It is also not recommended to use phenol red in your medium because it is a weak estrogen (PMID: 3458212). Cell stimulation is often conducted by dihydrotestosterone (DHT).

5. What is the role of AR in prostate cancer?

AR plays a key role in the development and physiology of the prostate gland, and also cancer progression (PMID: 15082523). Mutations in AR altering ligands have been observed. The progression of the prostate cancer depends on AR activity and therefore blocking AR activity or lowering androgen levels is a key step related to androgen deprivation therapy (ADT).


Protocols

Product Specific Protocols
WB protocol for androgen receptor antibody 22089-1-APDownload protocol
IHC protocol for androgen receptor antibody 22089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Med

The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy.

Authors - Zhi Cao
humanWB

Gene

miR-323-3p regulates the steroidogenesis and cell apoptosis in polycystic ovary syndrome (PCOS) by targeting IGF-1.

Authors - Tianjuan Wang
mouseWB

PLoS One

ETA-mediated anti-TNF-α therapy ameliorates the phenotype of PCOS model induced by letrozole.

Authors - Qin Lang
humanIHC

Onco Targets Ther

The Expression and Prognostic Significance of Claudin-8 and Androgen Receptor in Breast Cancer.

Authors - Yiqi Zhang
mouseIHC

Mol Hum Reprod

Melatonin protects the mouse testis against heat-induced damage.

Authors - Pengfei Zhang
humanWB

Acta Pharmacol Sin

RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.

Authors - Ying Ren

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Saswati Bhattacharya (08-20-2019)

This antibody worked in a cell line with a lower expression of AR, when another antibody from another vendor did not work. I am very pleased with its earlier lot. Also, since this is a polyclonal antibody, many bands light up, but in my opinion these are legitimate bands. Also hoping that the quality control keeps good eye from one lot to another., I have yet to use the particular lot# I put in this note. Thanks

  • Applications: Western Blot,Immunofluorescence,Flow Cytometry,
  • Primary Antibody Dilution: 1:6000
  • Cell Tissue Type: MDA-MB 231
No image submitted with review