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ARL13B Antibody 246 Publications

Rabbit Polyclonal| Catalog number: 17711-1-AP

Featured Product KD/KO validated

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2 customer reviews

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Con: 41 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ARL13B Antibody IF MDCK cells 17711-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ARL13B Antibody IF NIH3T3 cells 17711-1-AP

IF result of anti-ARL13B(17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

ARL13B Antibody IF hTERT-RPE1 17711-1-AP

IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

ARL13B Antibody IF mouse embryonic fibroblasts 17711-1-AP

IF result from Dr. Corbit, Kevin. anti-ARL13B(17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

ARL13B Antibody WB mouse liver tissue 17711-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ARL13B Antibody IP L02 cells 17711-1-AP

IP Result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

ARL13B Antibody IHC mouse brain tissue 17711-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ARL13B Antibody IHC mouse brain tissue 17711-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, dog, chicken, pig, sheep, Xenopus, Zebrafish
Positive WB detected in:
mouse liver tissue, mouse kidney tissue, L02 cells, NIH/3T3 cells, mouse brain tissue
Positive IP detected in:
L02 cells
Positive IHC detected in:
mouse brain tissue, human testis tissue, human liver tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MDCK cells, hTERT-RPE1, mouse embryonic fibroblasts, NIH3T3 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ARL13B fusion protein Ag12015
Full name:
ADP-ribosylation factor-like 13B
Calculated molecular weight:
48 kDa
Observed molecular weight:
40-48 kDa, 66 kDa
GenBank accession number:
BC094725
Gene ID (NCBI):
200894
Gene symbol
ARL13B
Synonyms
ARL13B, ARL2 like protein 1, ARL2L1, JBTS8

Background

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ARL13B Rabbit Polyclonal antibody

Lindsey (Verified Customer), 02-04-2019
Applications
Immunofluorescence,
Primary Antibody Dilution
1:1000
ARL13B Antibody Immunofluorescence, validation (1:1000 dilution) in E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells (Cat no:17711-1-AP)

ARL13B Antibody Immunofluorescence, validation (1:1000 dilution) in E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells (Cat no:17711-1-AP)

Cell/Tissue Type
E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells
Lot Number
Excellent specificity with minimal background/nonspecific binding; This antibody is ideal because it works just as well in embryonic mouse tissue as it does in adult. I couldn't recommend this product more!

ARL13B Rabbit Polyclonal antibody

Kishor (Verified Customer), 01-30-2019
Applications
Western Blot,Immunofluorescence,
Primary Antibody Dilution
1:1000 and 1:200
No image submitted with review

No image submitted with review

Cell/Tissue Type
H69, HUCCT-1 and Rat Liver tissue
Lot Number
I am very much satisfied with this produced, working good for WB (1:1000) and for IF (1:200)

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