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ARL13B Polyclonal antibody
ARL13B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, dog and More (6)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
328
Cat no : 17711-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, NIH/3T3 cells, L02 cells, mouse kidney tissue, rat liver tissue, mouse brain tissue |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | mouse brain tissue, human testis tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells, hTERT-RPE1, mouse embryonic fibroblasts, NIH3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 34 publications below |
| IHC | See 7 publications below |
| IF | See 300 publications below |
Product Information
17711-1-AP targets ARL13B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, dog samples.
| Tested Reactivity | human, mouse, rat, dog |
| Cited Reactivity | Chick, chicken, dog, human, mouse, pig, rat, sheep, swine, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARL13B fusion protein Ag12015 |
| Full Name | ADP-ribosylation factor-like 13B |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 40-48 kDa, 66 kDa |
| GenBank accession number | BC094725 |
| Gene symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL13B antibody 17711-1-AP | Download protocol |
| IHC protocol for ARL13B antibody 17711-1-AP | Download protocol |
| IF protocol for ARL13B antibody 17711-1-AP | Download protocol |
| IP protocol for ARL13B antibody 17711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Development Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines. | ||
Mol Cells Loss of Primary Cilia Results in the Development of Cancer in the Murine Thyroid Gland. | ||
Commun Biol Pharmacological cholesterol depletion disturbs ciliogenesis and ciliary function in developing zebrafish. | ||
Neuron HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH deng (Verified Customer) (01-30-2020) | Very good for cilia staining
 |
FH Gabriela (Verified Customer) (01-01-2020) | Very specific antibody for primary cilia. We use it in chicken embryos spinal cord.
|
FH KARTHIKEYAN (Verified Customer) (11-20-2019) | This antibody works really well in human and mouse cell lines and in zebrafish samples, we used this antibody for WB, IF and Flow cytometry.
 |
FH Rebeca (Verified Customer) (09-26-2019) | I have used this antibody for several immunofluorescence stainings of primary cilia. The antibody works beautifully, would definitely recommend!
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FH Pavel (Verified Customer) (09-18-2019) | Strong ciliary staining in MDCK cells. Works both with PFA and MeOH fixation.
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FH Lindsey (Verified Customer) (02-04-2019) | Excellent specificity with minimal background/nonspecific binding; This antibody is ideal because it works just as well in embryonic mouse tissue as it does in adult. I couldn't recommend this product more!
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FH Kishor (Verified Customer) (01-30-2019) | I am very much satisfied with this produced, working good for WB (1:1000) and for IF (1:200)
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