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ARL13B Antibody 189 Publications

Rabbit Polyclonal| Catalog number: 17711-1-AP

Featured Product KD/KO validated

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Con: 41 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ARL13B Antibody IF MDCK cells 17711-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed MDCK cells using 17711-1-AP(ARL13B antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ARL13B Antibody IF NIH3T3 cells 17711-1-AP

IF result of anti-ARL13B(17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

ARL13B Antibody IF hTERT-RPE1 17711-1-AP

IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

ARL13B Antibody IF mouse embryonic fibroblasts 17711-1-AP

IF result from Dr. Corbit, Kevin. anti-ARL13B(17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

ARL13B Antibody WB mouse liver tissue 17711-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ARL13B Antibody IP L02 cells 17711-1-AP

IP Result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

ARL13B Antibody IHC mouse brain tissue 17711-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ARL13B Antibody IHC mouse brain tissue 17711-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, dog, pig, sheep, Xenopus, Zebrafish
Positive WB detected in:
mouse liver tissue, mouse kidney tissue, L02 cells, NIH/3T3 cells, mouse brain tissue
Positive IP detected in:
L02 cells
Positive IHC detected in:
mouse brain tissue, human testis tissue, human liver tissue, human kidney tissue
Positive IF detected in:
MDCK cells, NIH3T3 cells, mouse embryonic fibroblasts, hTERT-RPE1
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ARL13B fusion protein Ag12015
Full name:
ADP-ribosylation factor-like 13B
Calculated molecular weight:
48 kDa
Observed molecular weight:
40-48 kDa, 66 kDa
GenBank accession number:
BC094725
Gene ID (NCBI):
200894
Gene symbol
ARL13B
Synonyms
ARL13B, ARL2 like protein 1, ARL2L1, JBTS8

Background

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).


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