Name: ARL13B Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

ARL13B Antibody IF MDCK cells 17711-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ARL13B Antibody IF NIH3T3 cells 17711-1-AP

IF result of anti-ARL13B(17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

ARL13B Antibody IF hTERT-RPE1 17711-1-AP

IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

ARL13B Antibody IF mouse embryonic fibroblasts 17711-1-AP

IF result from Dr. Corbit, Kevin. anti-ARL13B(17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

ARL13B Antibody WB mouse liver tissue 17711-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IP Result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

ARL13B Antibody IHC mouse brain tissue 17711-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:

human, mouse, rat, dog, chicken, pig, sheep, Xenopus, Zebrafish

Positive WB detected in:

mouse liver tissue, mouse kidney tissue, L02 cells, NIH/3T3 cells, mouse brain tissue

Positive IP detected in:

L02 cells

Positive IHC detected in:

mouse brain tissue, human testis tissue, human liver tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

MDCK cells, hTERT-RPE1, mouse embryonic fibroblasts, NIH3T3 cells

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ARL13B fusion protein Ag12015

Full name:

ADP-ribosylation factor-like 13B

Calculated molecular weight:

48 kDa

Observed molecular weight:

40-48 kDa, 66 kDa

GenBank accession number:

BC094725

Gene ID (NCBI):

200894

Gene symbol

ARL13B

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, JBTS8

Background

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two speciﬁc bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ARL13B Rabbit Polyclonal antibody

Lindsey (Verified Customer), 02-04-2019

Applications

Immunofluorescence,

Primary Antibody Dilution

1:1000

ARL13B Antibody Immunofluorescence, validation (1:1000 dilution) in E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells (Cat no:17711-1-AP)

Cell/Tissue Type

E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells

Lot Number

Excellent specificity with minimal background/nonspecific binding; This antibody is ideal because it works just as well in embryonic mouse tissue as it does in adult. I couldn't recommend this product more!

ARL13B Rabbit Polyclonal antibody

Kishor (Verified Customer), 01-30-2019

Applications

Western Blot,Immunofluorescence,

Primary Antibody Dilution

1:1000 and 1:200

No image submitted with review

Cell/Tissue Type

H69, HUCCT-1 and Rat Liver tissue

Lot Number

I am very much satisfied with this produced, working good for WB (1:1000) and for IF (1:200)

Showing 1- [6] of [225] Publications

Author:		Humbert Melissa C MC
Pubmed ID:		23150559
Journal:		Proc Natl Acad Sci U S A
Title:		ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.
Application:		WB,IF
KD validated
Species:		mouse, human

Author:		Stephen D Thorpe
Pubmed ID:		28158906
Journal:		J Cell Physiol
Title:		Reduced Primary Cilia Length and Altered Arl13b Expression Are Associated with Deregulated Chondrocyte Hedgehog Signalling in Alkaptonuria.
Application:		IF
KD validated
Species:		human

Author:		Ping Song
Pubmed ID:		27571019
Journal:		Invest Ophthalmol Vis Sci
Title:		Arl13b Interacts With Vangl2 to Regulate Cilia and Photoreceptor Outer Segment Length in Zebrafish.
Application:		WB
KD validated
Species:		human

Author:		Kai He
Pubmed ID:		30120249
Journal:		Nat Commun
Title:		Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.
Application:		WB,IF
KD validated
Species:		human

Author:		Yuanyuan Li
Pubmed ID:		27153923
Journal:		J Am Soc Nephrol
Title:		Deletion of ADP Ribosylation Factor-Like GTPase 13B Leads to Kidney Cysts.
Application:		IF
KO validated
Species:		mouse

Author:		Christin Hanke-Gogokhia
Pubmed ID:		29089384
Journal:		J Biol Chem
Title:		The guanine nucleotide exchange factor, Arf-like protein 13b, is essential for assembly of the mouse photoreceptor transition zone and outer segment.
Application:		WB,IF
KO validated
Species:		mouse

WB Figures

L02 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ARL13B Antibody WB mouse brain tissue 17711-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

ARL13B Antibody WB mouse kidney tissue 17711-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ARL13B Antibody WB NIH/3T3 cells 17711-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP(ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

IHC Figures

ARL13B Antibody IHC human kidney tissue 17711-1-AP

Immunohistochemical of paraffin-embedded human kidney using 17711-1-AP(ARL13B antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using 17711-1-AP(ARL13B antibody) at dilution of 1:50 (under 40x lens)

ARL13B Antibody IHC human liver tissue 17711-1-AP

Immunohistochemical of paraffin-embedded human liver using 17711-1-AP(ARL13B antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver using 17711-1-AP(ARL13B antibody) at dilution of 1:50 (under 40x lens)

ARL13B Antibody IHC human testis tissue 17711-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human testis tissue slide using 17711-1-AP( ARL13B Antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

IF Figures

IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

Immunofluorescent analysis of ( 4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( 4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent images of MDCK cells stained with ARL13B rabbit pAb (17711-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Rabbit IgG(H+L) for 17711-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 66200-1-Ig.

IF result from Dr. Corbit, Kevin. anti-ARL13B(17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

IF result of anti-ARL13B(17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

ARL13B Antibody 225 Publications

Rabbit Polyclonal| Catalog number: 17711-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

ARL13B Rabbit Polyclonal antibody

ARL13B Rabbit Polyclonal antibody

Showing 1- [6] of [225] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

ARL13B Antibody 225 Publications

Rabbit Polyclonal| Catalog number: 17711-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Customer Reviews

ARL13B Rabbit Polyclonal antibody

ARL13B Rabbit Polyclonal antibody