CoraLite® Plus 647-conjugated ARL13B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL13B Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-17711

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

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Validation Data Gallery

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  IF Staining of MDCK using CL647-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 647-conjugated ARL13B antibody (CL647-17711) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL647-17711 targets ARL13B in IF applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 40-48 kDa, 66 kDa
• GenBank accession number: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 665 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).