CoraLite® Plus 647-conjugated ARL13B Polyclonal antibody

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  IF Staining of MDCK using CL647-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 647 ARL13B antibody (CL647-17711) at dilution of 1:200, Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

• 

  IF Staining of MDCK using CL647-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 647-conjugated ARL13B antibody (CL647-17711) at dilution of 1:100.

• Featured Product
• KD/KO Validated

ARL13B Polyclonal Antibody for IF/ICC

Cat No. CL647-17711

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

IF/ICC

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL647-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 40-48 kDa, 66 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• RRID: AB_2920234
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3SXY8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Publications

Species	Application	Title
rat	IF	bioRxiv; Convergence of autism proteins at the cilium; Authors - Elina Kostyanovskaya
	IF	Cell Rep; Selective targeting of mu opioid receptors to primary cilia; Authors - Rita R Fagan