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CoraLite® Plus 647-conjugated ARL13B Polyclonal antibody
ARL13B Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
IF
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-17711
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MDCK cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL647-17711 targets ARL13B in IF applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ARL13B fusion protein Ag12015 |
Full Name | ADP-ribosylation factor-like 13B |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 40-48 kDa, 66 kDa |
GenBank accession number | BC094725 |
Gene symbol | ARL13B |
Gene ID (NCBI) | 200894 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 650 nm / 665 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 ARL13B antibody CL647-17711 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |