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CoraLite®647-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF

Conjugate

CoraLite®647 Fluorescent Dye

Cat no : CL647-17711

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8



Tested Applications

Positive IF detected inMDCK cells
For other applications, we recommend the unconjugated version of this antibody, 17711-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL647-17711 targets ARL13B in IF applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12015
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 40-48 kDa, 66 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
IF protocol for CL647 ARL13B antibody CL647-17711Download protocol
Standard Protocols
Click here to view our Standard Protocols