Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Calu-1 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5238 Product name: Recombinant human ARNT,HIF1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC060838 Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS Predict reactive species |
| Full Name | aryl hydrocarbon receptor nuclear translocator |
| Calculated Molecular Weight | 416 aa, 46 kDa, 87 kDa |
| Observed Molecular Weight | 87 kDa |
| GenBank Accession Number | BC060838 |
| Gene Symbol | ARNT/HIF-1 beta |
| Gene ID (NCBI) | 405 |
| RRID | AB_10639523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27540 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| IHC protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| IP protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| WB protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARNT/HIF-1β polyclonal antibody (Cat# 14105-1-AP) has accumulated 33 citations published in journals such as Nature Communications, Hepatology, PNAS, Advanced Science, Redox Biology, Phytomedicine, Free Radical Biology and Medicine, and Journal of Investigative Dermatology. Associated research fields include hypoxia/HIF signaling, aryl hydrocarbon receptor pathways, oncology (liver, lung, esophageal, breast, renal cancers), inflammation, bone regeneration, cardiac hypertrophy, neuroprotection, ferroptosis, environmental toxicology, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Bioact Mater Mantis shrimp saddle-mimetic amorphous calcium (zinc) phosphate/chitin scaffolds with superior mechanical properties and bioactivity for bone regeneration. | ||
Nat Commun dFLASH; dual FLuorescent transcription factor activity sensor for histone integrated live-cell reporting and high-content screening | ||
Hepatology PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability. | ||
Redox Biol USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2 | ||
Acta Pharm Sin B Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway | ||
Adv Sci (Weinh) CA9-Targeted PET Imaging for Noninvasive Discrimination of Clear Cell Renal Cell Carcinoma and Associated Tumor Biological Features |
