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ARNT,HIF1B Polyclonal antibody
ARNT,HIF1B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 14105-1-AP
|Positive WB detected in||HepG2 cells, HeLa cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||mouse embryo tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14105-1-AP targets ARNT,HIF1B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARNT,HIF1B fusion protein Ag5238|
|Full Name||aryl hydrocarbon receptor nuclear translocator|
|Calculated molecular weight||416 aa, 46 kDa, 87 kDa|
|Observed molecular weight||86 kDa|
|GenBank accession number||BC060838|
|Gene ID (NCBI)||405|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia
|Product Specific Protocols|
|WB protocol for ARNT,HIF1B antibody 14105-1-AP||Download protocol|
|IHC protocol for ARNT,HIF1B antibody 14105-1-AP||Download protocol|
|IF protocol for ARNT,HIF1B antibody 14105-1-AP||Download protocol|
|IP protocol for ARNT,HIF1B antibody 14105-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-c-src-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis.
Biol Pharm Bull
Isorhamnetin Inhibits Reactive Oxygen Species-Dependent Hypoxia Inducible Factor (HIF)-1α Accumulation.
Int J Mol Med
Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes.
J Interferon Cytokine Res
Interferon-γ-Induced Intestinal Epithelial Barrier Dysfunction by NF-κB/HIF-1α Pathway.
J Biomed Nanotechnol
Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.
Free Radic Biol Med
Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.