Back

ATF2 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 11908-1-AP

Be the first to review this product

-_-

Freight/Packing

Con: 52 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IP, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ATF2 Antibody IP mouse brain tissue 11908-1-AP

IP result of anti-ATF2(IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug

ATF2 Antibody WB HeLa cells 11908-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 11908-1-AP(ATF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ATF2 Antibody IF HeLa cells 11908-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11908-1-AP(ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, mouse brain tissue
Positive IP detected in:
mouse brain tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATF2 fusion protein Ag2581
Full name:
activating transcription factor 2
Calculated molecular weight:
209aa,23 kDa
Observed molecular weight:
60 kDa,64 kDa
GenBank accession number:
BC026175
Gene ID (NCBI):
1386
Gene symbol
ATF2
Synonyms
ATF2, CRE BP1, CREB 2, CREB2, CREBP1, HB16, TREB7

Background

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).


Back
to top