ATF5 Monoclonal antibody

ATF5 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1E5B6

Cat no : 67066-1-Ig

Synonyms

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx



Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

67066-1-Ig targets ATF5 in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATF5 fusion protein Ag7214
Full Name activating transcription factor 5
Calculated molecular weight 31 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC005174
Gene symbol ATF5
Gene ID (NCBI) 22809
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).

Protocols

Product Specific Protocols
WB protocol for ATF5 antibody 67066-1-IgDownload protocol
Standard Protocols
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