Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67066-1-Ig targets ATF5 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7214 Product name: Recombinant human ATF5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-282 aa of BC005174 Sequence: MSLLATLGLELDRALLPASGLGWLVDYGKLPPAPAPLAPYEVLGGALEGGLPVGGEPLAGDGFSDWMTERVDFTALLPLEPPLPPGTLPQPSPTPPDLEAMASLLKKELEQMEDFFLDAPLLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDLLAIYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC Predict reactive species |
| Full Name | activating transcription factor 5 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC005174 |
| Gene Symbol | ATF5 |
| Gene ID (NCBI) | 22809 |
| RRID | AB_2882376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y2D1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATF5 antibody 67066-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ATF5 Monoclonal antibody (1E5B6), Cat# 67066-1-Ig, has 11 citations in journals including Cell Metabolism, Nature Communications, EMBO Molecular Medicine, Stem Cell Research & Therapy, Int J Mol Sci, Toxicology Letters, FEBS Open Bio, J Muscle Res Cell Motil, World J Surg Oncol, and NPJ Dementia. Research fields span mitochondrial stress/dysfunction, UPRmt signaling, exercise physiology, cardiovascular disease, cancer, intervertebral disc degeneration, muscle aging, and neurodegeneration.
| Species | Application | Title |
|---|---|---|
Cell Metab Excessive vigorous exercise impairs cognitive function through a muscle-derived mitochondrial pretender. | ||
Nat Commun Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice | ||
EMBO Mol Med ER stress induced mitochondrial dysfunction drives Treg instability in coronary artery disease. | ||
Stem Cell Res Ther From collapse to comeback: Luteolin rejuvenates nucleus pulposus progenitor cells via SIRT1/ATF5-UPRmt to reverse intervertebral disc degeneration cascade. | ||
Int J Mol Sci The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. | ||
Toxicol Lett Bavachin induces liver injury and cell apoptosis by targeting Wnt/β-catenin/DRP1 signaling pathway mediated mitochondrial dysfunction |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. One user found it performed well on human-iPSC-derived immune cells at a 1:5000 dilution. The other, working with HCT116 cells, noted a couple of aspecific bands at higher molecular weight but confirmed the target band was significantly brighter and clearly distinguishable. Overall, users report reliable specificity and strong signal for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christin (Verified Customer) (12-22-2025) | Good antibody for western blot
|
Morgane (Verified Customer) (09-22-2025) | A couple aspecific bands in higher MW but the corresponding one is much brighter.
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