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  • Featured Product
  • KD/KO Validated

ATL2 Polyclonal antibody

ATL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16688-1-AP

Synonyms

Aip 2, ARL 6 interacting protein 2, ARL3IP2, ARL6IP2, ATL2, Atlastin 2, atlastin GTPase 2, atlastin2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATL2 Polyclonal antibody (16688-1-AP)

    WB analysis using 16688-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ATL2 Polyclonal antibody (16688-1-AP)

    WB analysis of Jurkat using 16688-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATL2 Polyclonal antibody (16688-1-AP)

    WB analysis of HeLa using 16688-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16688-1-AP (ATL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16688-1-AP targets ATL2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATL2 fusion protein Ag10030
Full Name atlastin GTPase 2
Calculated Molecular Weight412aa,47 kDa; 583aa,66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC053508
Gene Symbol ATL2
Gene ID (NCBI) 64225
RRIDAB_1850898
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATL2 antibody 16688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Cell Biol

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Authors - Nan Liu
View Article
human,mouse,monkeyWB

J Cell Biol

Human atlastin-3 is a constitutive ER membrane fusion catalyst

Authors - Samantha Bryce
View Article
mouseWB

Nature

Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy

Authors - Hector Foronda
View Article
humanWB,IF

J Biochem

Lunapark ubiquitinates atlastin-2 for the tubular network formation of the endoplasmic reticulum.

Authors - Toshiaki Sakisaka
View Article
mouseWB

NPJ Parkinsons Dis

Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities

Authors - Jia Yu
View Article
monkeyWB

Curr Biol

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

Authors - Qingzhou Chen
  • KO Validated
View Article