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ATP1A3 Polyclonal antibody

ATP1A3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25727-1-AP

Synonyms

ATP1A3, DYT12, RDP, Sodium pump subunit alpha 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A3 Polyclonal antibody (25727-1-AP)

    WB analysis using 25727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25727-1-AP (ATP1A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of rat brain tissue using ATP1A3 Polyclonal antibody (25727-1-AP)

    IP experiment of rat brain using 25727-1-AP

    IP result of anti-ATP1A3 (IP:25727-1-AP, 4ug; Detection:25727-1-AP 1:500) with rat brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1A3 Polyclonal antibody (25727-1-AP)

    IHC staining of human prostate cancer using 25727-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ATP1A3 Polyclonal antibody (25727-1-AP)

    IHC staining of human prostate cancer using 25727-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ATP1A3 Polyclonal antibody (25727-1-AP)

    IF Staining of mouse brain using 25727-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ATP1A3 Polyclonal antibody (25727-1-AP)

    IF Staining of mouse brain using 25727-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25727-1-AP targets ATP1A3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A3 fusion protein Ag22842
Full Name ATPase, Na+/K+ transporting, alpha 3 polypeptide
Calculated Molecular Weight 1013 aa, 112 kDa
Observed Molecular Weight110-113 kDa
GenBank Accession NumberBC015566
Gene Symbol ATP1A3
Gene ID (NCBI) 478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na/K homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

Protocols

Product Specific Protocols
WB protocol for ATP1A3 antibody 25727-1-APDownload protocol
IHC protocol for ATP1A3 antibody 25727-1-APDownload protocol
IF protocol for ATP1A3 antibody 25727-1-APDownload protocol
IP protocol for ATP1A3 antibody 25727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols