ATP2A1 Polyclonal antibody

ATP2A1 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22361-1-AP

Synonyms

ATP2A, ATP2A1, Calcium pump 1, SERCA1, SR Ca(2+) ATPase 1



Tested Applications

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22361-1-AP is ATP2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP2A1 fusion protein Ag17944
Full Name ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Calculated molecular weight 1001 aa, 110 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC037354
Gene symbol ATP2A1
Gene ID (NCBI) 487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ATP2A1 antibody 22361-1-APDownload protocol
IP protocol for ATP2A1 antibody 22361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Ther Nucleic Acids

lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.

Authors - Jie Yang
mouseWB

J Muscle Res Cell Motil

Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.

Authors - Nagakatsu Harada