ATP6V0A1 Polyclonal antibody

ATP6V0A1 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13828-1-AP

Synonyms

a1, ATP6N1, ATP6N1A, ATP6V0A1, Stv1, V ATPase 116 kDa isoform a1, Vacuolar proton pump subunit 1, Vph1, VPP1



Tested Applications

Positive IP detected inmouse brain tissue,
Positive IHC detected inmouse brain tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13828-1-AP targets ATP6V0A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V0A1 fusion protein Ag4191
Full Name ATPase, H+ transporting, lysosomal V0 subunit a1
Calculated molecular weight 837 aa, 96 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC032398
Gene symbol ATP6V0A1
Gene ID (NCBI) 535
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ATP6V0A1 antibody 13828-1-APDownload protocol
IP protocol for ATP6V0A1 antibody 13828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Physiol

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Authors - Vishwanatha K Rao