ATP6V1B2 Polyclonal antibody

ATP6V1B2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15097-1-AP

Synonyms

ATP6B1B2, ATP6B2, ATP6V1B2, HO57, V ATPase subunit B 2, VATB, Vma2, VPP3



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, HeLa cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

15097-1-AP targets ATP6V1B2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1B2 fusion protein Ag7174
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Calculated molecular weight 57 kDa
Observed molecular weight 56-58 kDa
GenBank accession numberBC003100
Gene symbol ATP6V1B2
Gene ID (NCBI) 526
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

Protocols

Product Specific Protocols
WB protocol for ATP6V1B2 antibody 15097-1-APDownload protocol
IHC protocol for ATP6V1B2 antibody 15097-1-APDownload protocol
IP protocol for ATP6V1B2 antibody 15097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.

Authors - Milena Kluge
mouseWB

Synapse

Proteomic identification of synaptic caspase substrates.

Authors - Ken G Victor
humanWB

Vet Res

Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Authors - Bingxin Chu
humanWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle