ATP6V1G2 Polyclonal antibody

ATP6V1G2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25316-1-AP

Synonyms

ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10



Tested Applications

Positive WB detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 25316-1-AP is ATP6V1G2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1G2 fusion protein Ag18007
Full Name ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2
Calculated molecular weight 118 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC119726
Gene symbol ATP6V1G2
Gene ID (NCBI) 534
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
IHC protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.

Authors - Irene Bertolini
humanWB

EBioMedicine

Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.

Authors - Andrea Terrasi
humanWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle