CD80/B7-1 Antibody
CD80/B7-1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1E2F10
Cat no : 66406-1-Ig
Synonyms
Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CTLA 4 counter receptor B7.1, LAB7
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Daudi cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
66406-1-Ig targets CD80/B7-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD80/B7-1 fusion protein Ag5615 |
| Full Name | CD80 molecule |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC042665 |
| Gene symbol | CD80 |
| Gene ID (NCBI) | 941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD80/B7-1 antibody 66406-1-Ig | Download protocol |
| IHC protocol for CD80/B7-1 antibody 66406-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun The LncRNA MALAT1 regulates CD80 transcription via the NF-κB signaling pathway in the A549 cell line. | ||
Int J Biochem Cell Biol Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome. | ||
Pathol Oncol Res The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets. |
