CD80/B7-1 Monoclonal antibody
CD80/B7-1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1E2F10
Cat no : 66406-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Daudi cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
66406-1-Ig targets CD80/B7-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD80/B7-1 fusion protein Ag5615 |
| Full Name | CD80 molecule |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC042665 |
| Gene symbol | CD80 |
| Gene ID (NCBI) | 941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD80/B7-1 antibody 66406-1-Ig | Download protocol |
| IHC protocol for CD80/B7-1 antibody 66406-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun The LncRNA MALAT1 regulates CD80 transcription via the NF-κB signaling pathway in the A549 cell line. | ||
Int J Biochem Cell Biol Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome. | ||
Cell Death Dis TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca2+/CaMKII/Nrf2 signaling pathway. | ||
ACS Appl Mater Interfaces Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy. | ||
Pathol Oncol Res The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets. |
