CD80/B7-1 Monoclonal antibody

CD80/B7-1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E2F10

Cat no : 66406-1-Ig

Synonyms

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CD80/B7-1, CTLA 4 counter receptor B7.1, LAB7



Tested Applications

Positive WB detected inRaji cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66406-1-Ig targets CD80/B7-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD80/B7-1 fusion protein Ag5615
Full Name CD80 molecule
Calculated molecular weight 33 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC042665
Gene symbol CD80
Gene ID (NCBI) 941
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
WB protocol for CD80/B7-1 antibody 66406-1-IgDownload protocol
IHC protocol for CD80/B7-1 antibody 66406-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Photodiagnosis Photodyn Ther

PMID: 30902794

Authors - Jie Ji
humanWB

Biochem Biophys Res Commun

The LncRNA MALAT1 regulates CD80 transcription via the NF-κB signaling pathway in the A549 cell line.

Authors - Chenxia Juan
humanWB

Int J Biochem Cell Biol

Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.

Authors - Chenxia Juan
ratIF

Cell Death Dis

TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca2+/CaMKII/Nrf2 signaling pathway.

Authors - Zhongyang Lv
mouseWB

ACS Appl Mater Interfaces

Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.

Authors - Jiaojiao Zhu
humanWB

Pathol Oncol Res

The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets.

Authors - Yuanhang Li