CD80 Monoclonal antibody, PBS Only

• 

  WB analysis of DC2.4 using 66406-1-Ig (same clone as 66406-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66406-1-Ig (same clone as 66406-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66406-1-Ig (same clone as 66406-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

CD80 Monoclonal Antibody for WB, ELISA

Cat No. 66406-1-PBS

Clone No.1E2F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, ELISA

Synonyms

1E2F10, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

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Product Information

66406-1-PBS targets CD80 in WB, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5615

  Product name: Recombinant human B7-1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 36-228 aa of BC042665

  Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT

• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC042665
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_2827408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P33681
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)