BAD Polyclonal antibody

BAD Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10435-1-AP

Synonyms

BAD, BBC2, BBC6



Tested Applications

Positive WB detected inJurkat cells, Tunicamycin treated HeLa cells
Positive IHC detected inhuman prostate hyperplasia tissue, human lymphoma tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Sample-dependent, check data in validation data gallery

Product Information

10435-1-AP targets BAD in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAD fusion protein Ag0688
Full Name BCL2-associated agonist of cell death
Calculated molecular weight 18 kDa
Observed molecular weight 18-25 kDa
GenBank accession numberBC001901
Gene symbol BAD
Gene ID (NCBI) 572
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Protocols

Product Specific Protocols
WB protocol for BAD antibody 10435-1-APDownload protocol
IHC protocol for BAD antibody 10435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Transl Neurosci

PMID: 30746281

Authors - Guoqing Zhao
humanWB

J Enzyme Inhib Med Chem

Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.

Authors - Feifei Qin
humanWB

Cancer Lett

A novel hydrogen sulfide-releasing donor, HA-ADT, suppresses the growth of human breast cancer cells through inhibiting the PI3K/AKT/mTOR and Ras/Raf/MEK/ERK signaling pathways.

Authors - Qian Dong
humanWB

PLoS One

The ginsenoside Rk3 exerts anti-esophageal cancer activity in vitro and in vivo by mediating apoptosis and autophagy through regulation of the PI3K/Akt/mTOR pathway.

Authors - Huanhuan Liu
humanWB

Int J Nanomedicine

Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.

Authors - Muhammad Waseem Khan
humanWB

Biomolecules

Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells.

Authors - Minjing Li