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  • Featured Product
  • KD/KO Validated

BBS10 Polyclonal antibody

BBS10 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 12421-2-AP

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of HepG2 using 12421-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of A549 using 12421-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of MCF-7 using 12421-2-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS10 Polyclonal antibody (12421-2-AP)

    IF Staining of hTERT-RPE1 cells using 12421-2-AP

    IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim.

Tested Applications

Positive WB detected inHepG2 cells, A549 cells, MCF-7 cells
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12421-2-AP targets BBS10 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS10 fusion protein Ag3073
Full Name Bardet-Biedl syndrome 10
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013795
Gene Symbol BBS10
Gene ID (NCBI) 79738
RRIDAB_2064962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

Protocols

Product Specific Protocols
WB protocol for BBS10 antibody 12421-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation.

Authors - Marion Vincent V
  • KD Validated
View Article
human,mouseWB,IF

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S
View Article
mouseWB

Cilia

Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.

Authors - Noëlle Cognard
  • KO Validated
View Article