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Tested Applications
| Positive WB detected in | A549 cells, K-562 cells, Jurkat cells, Raji cells, Ramos cells, mouse ovary tissue, rat ovary tissue |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
50180-1-AP targets BCL10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0037 Product name: Recombinant human BCL10 protein Source: e coli.-derived, Unknow Tag: N/A Domain: BCL10 of BC053617 Sequence: Predict reactive species |
| Full Name | B-cell CLL/lymphoma 10 |
| Calculated Molecular Weight | 233 aa, 26 kDa |
| Observed Molecular Weight | 26-32 kDa |
| GenBank Accession Number | BC053617 |
| Gene Symbol | BCL10 |
| Gene ID (NCBI) | 8915 |
| RRID | AB_2243501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95999 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BCL10 antibody 50180-1-AP | Download protocol |
| IHC protocol for BCL10 antibody 50180-1-AP | Download protocol |
| WB protocol for BCL10 antibody 50180-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem The IRAK-1-BCL10-MALT1-TRAF6-TAK1 cascade mediates signaling to NF-kappaB from Toll-like receptor 4. | ||
J Biol Chem BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2.
| ||
Cell Signal The paracaspase MALT1 is a downstream target of Smad3 and potentiates the crosstalk between TGF-β and NF-kB signaling pathways in cancer cells |
