BTD Polyclonal antibody

BTD Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16330-1-AP

Synonyms

Biotinase, biotinidase, BTD



Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16330-1-AP targets BTD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTD fusion protein Ag9449
Full Name biotinidase
Calculated molecular weight 543 aa, 61 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC012099
Gene symbol BTD
Gene ID (NCBI) 686
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BTD antibody 16330-1-APDownload protocol
IHC protocol for BTD antibody 16330-1-APDownload protocol
IF protocol for BTD antibody 16330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols