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BTN3A1 Polyclonal antibody
BTN3A1 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC
Conjugate
Unconjugated
Cat no : 25221-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 25221-1-AP is BTN3A1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BTN3A1 fusion protein Ag18414 |
| Full Name | butyrophilin, subfamily 3, member A1 |
| Calculated Molecular Weight | 513 aa, 58 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC121800 |
| Gene Symbol | BTN3A1 |
| Gene ID (NCBI) | 11119 |
| RRID | AB_2879969 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BTN3A1, also named as Butyrophilin subfamily 3 member A1 or BTF5, is a 513 amino acid protein ,which contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 localizes in the cell membrane and belongs to the immunoglobulin superfamily. BTN/MOG family. BTN3A1 is detected on T-cells, natural killer cells, dendritic cells and macrophages. It is ubiquitously highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. It also regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. It exists as four isoforms. We detected a 48 kda isoform by western blot.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BTN3A1 antibody 25221-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.
| ||
Front Oncol Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer. | ||
Biochem Biophys Res Commun Expression level of BTN3A1 on the surface of CD14+ monocytes is a potential predictor of γδ T cell expansion efficiency. | ||
FEBS Open Bio Comprehensive analysis of BTN3A1 in cancers: mining of omics data and validation in patient samples and cellular models. |
