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C4orf29 Polyclonal antibody

C4orf29 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25036-1-AP

Synonyms

C4orf29

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C4orf29 Polyclonal antibody (25036-1-AP)

    WB analysis of PC-3 using 25036-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using C4orf29 Polyclonal antibody (25036-1-AP)

    WB analysis of A549 using 25036-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using C4orf29 Polyclonal antibody (25036-1-AP)

    WB analysis of mouse heart using 25036-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C4orf29 Polyclonal antibody (25036-1-AP)

    IHC staining of human liver using 25036-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using C4orf29 Polyclonal antibody (25036-1-AP)

    IHC staining of human liver using 25036-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C4orf29 Polyclonal antibody (25036-1-AP)

    IF Staining of A549 using 25036-1-AP

    Immunofluorescent analysis of A549 cells using 25036-1-AP (C4orf29 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, A549 cells, mouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25036-1-AP is C4orf29 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C4orf29 fusion protein Ag19120
Full Name chromosome 4 open reading frame 29
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC128143
Gene Symbol C4orf29
Gene ID (NCBI) 80167
RRIDAB_2879862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C4orf29 (Chromosome 4 open reading frame 29) is a secreted protein in humans that is encoded by the C4orf29 gene which maps to human chromosome 4. The function of C4orf29 is uncharacterised.

Protocols

Product Specific Protocols
WB protocol for C4orf29 antibody 25036-1-APDownload protocol
IHC protocol for C4orf29 antibody 25036-1-APDownload protocol
IF protocol for C4orf29 antibody 25036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols