C5aR Antibody

C5aR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21316-1-AP

Synonyms

C5A, C5a R, C5aR, C5AR1, C5R1, CD88



Tested Applications

Positive WB detected inPC-3 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman tonsillitis tissue, human lung tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The 21316-1-AP immunogen is Fusion Protein C5aR protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5aR fusion protein Ag15968
Full Name complement component 5a receptor 1
Calculated molecular weight 350aa,39 kDa
Observed molecular weight 45-50 kDa
GenBank accession number BC008982
Gene symbol C5AR1
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C5aR, also named as CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

Protocols

Product Specific Protocols
WB protocol for C5aR antibody 21316-1-APDownload protocol
IHC protocol for C5aR antibody 21316-1-APDownload protocol
IF protocol for C5aR antibody 21316-1-APDownload protocol
IP protocol for C5aR antibody 21316-1-APDownload protocol
FC protocol for C5aR antibody 21316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Neural Regen Res

RNA sequencing screening of differentially expressed genes after spinal cord injury.

Authors - Yi Li
mouseWB,IHC,IF

Biomed Res Int

N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor.

Authors - Shuai Huang
humanWB

J Immunol Res

Linking Complement C3 and B Cells in Nasal Polyposis.

Authors - Ulrike Werner
swineWB

PLoS One

Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.

Authors - Birte Weber
humanWB

Int J Mol Med

Time-dependent C5a and C5aR expression in dental pulp cells following stimulation with LTA and LPS.

Authors - Mingyue Liu
ratWB

PLoS One

Experimental blunt chest trauma-induced myocardial inflammation and alteration of gap-junction protein connexin 43.

Authors - Miriam Kalbitz