Validation Data Gallery
|Positive WB detected in||PC-3 cells, L02 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human tonsillitis tissue, human lung tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells, PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The 21316-1-AP immunogen is Fusion Protein C5aR protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C5aR fusion protein Ag15968|
|Full Name||complement component 5a receptor 1|
|Calculated molecular weight||350aa,39 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC008982|
|Gene ID (NCBI)||728|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
C5aR, also named as CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).
|Product Specific Protocols|
|WB protocol for C5aR antibody 21316-1-AP||Download protocol|
|IHC protocol for C5aR antibody 21316-1-AP||Download protocol|
|IF protocol for C5aR antibody 21316-1-AP||Download protocol|
|IP protocol for C5aR antibody 21316-1-AP||Download protocol|
|FC protocol for C5aR antibody 21316-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Neural Regen Res
RNA sequencing screening of differentially expressed genes after spinal cord injury.
Biomed Res Int
N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor.
J Immunol Res
Linking Complement C3 and B Cells in Nasal Polyposis.
Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.
Int J Mol Med
Time-dependent C5a and C5aR expression in dental pulp cells following stimulation with LTA and LPS.
Experimental blunt chest trauma-induced myocardial inflammation and alteration of gap-junction protein connexin 43.