Calretinin Polyclonal antibody
Calretinin Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 12278-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, human brain tissue, human colon tissue, human ileum tissue, mouse colon tissue, rat brain tissue |
Positive IHC detected in | human appendicitis tissue, human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 2 publications below |
Product Information
12278-1-AP targets Calretinin in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Calretinin fusion protein Ag2924 |
Full Name | calbindin 2 |
Calculated molecular weight | 29 kDa |
Observed molecular weight | 29 kDa |
GenBank accession number | BC015484 |
Gene symbol | CALB2 |
Gene ID (NCBI) | 794 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.
Protocols
Product Specific Protocols | |
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WB protocol for Calretinin antibody 12278-1-AP | Download protocol |
IHC protocol for Calretinin antibody 12278-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Pediatr Res Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2. | ||
Am J Transl Res Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions. | ||
Endocrinology Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persist in Aged Offspring. |