Calretinin Polyclonal antibody
Calretinin Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12278-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, human brain tissue, human colon tissue, human ileum tissue, mouse colon tissue, rat brain tissue |
| Positive IHC detected in | human appendicitis tissue, human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
12278-1-AP targets Calretinin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Calretinin fusion protein Ag2924 |
| Full Name | calbindin 2 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC015484 |
| Gene symbol | CALB2 |
| Gene ID (NCBI) | 794 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Calretinin antibody 12278-1-AP | Download protocol |
| IHC protocol for Calretinin antibody 12278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pediatr Res Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2. | ||
Am J Transl Res Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions. | ||
Endocrinology Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persist in Aged Offspring. |
