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Calretinin Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 12278-1-AP

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Calretinin Antibody IHC human appendicitis tissue 12278-1-AP

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Calretinin Antibody IHC human appendicitis tissue 12278-1-AP

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Calretinin Antibody WB fetal human brain tissue 12278-1-AP

fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP( Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
fetal human brain tissue, human brain tissue, human colon tissue, human ileum tissue, mouse colon tissue, rat brain tissue
Positive IHC detected in:
human appendicitis tissue, human brain tissue, human cerebellum tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:400-1:1600
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Calretinin fusion protein Ag2924
Full name:
calbindin 2
Calculated molecular weight:
29 kDa
Observed molecular weight:
29 kDa
GenBank accession number:
BC015484
Gene ID (NCBI):
794
Gene symbol
CALB2
Synonyms
29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

Background

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.


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