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CARM1 Polyclonal antibody
CARM1 Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 55246-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
55246-1-AP targets CARM1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | coactivator-associated arginine methyltransferase 1 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 63-66 kDa, 55 kDa |
| GenBank Accession Number | NM_199141 |
| Gene Symbol | CARM1 |
| Gene ID (NCBI) | 10498 |
| RRID | AB_10863291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CARM1 antibody 55246-1-AP | Download protocol |
| IHC protocol for CARM1 antibody 55246-1-AP | Download protocol |
| IF protocol for CARM1 antibody 55246-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Chem Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA | ||
Eur J Med Chem Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. | ||
Front Cell Dev Biol Osteocytes Enhance Osteogenesis by Autophagy-Mediated FGF23 Secretion Under Mechanical Tension. | ||
Cancer Cell Int CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.
| ||
RSC Adv Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression | ||
J Reprod Immunol Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH fatemeh (Verified Customer) (07-25-2022) | western blot was done on 50 ug of cell lysate. Blot was transfered using wet transfer method (300 mA for 90 min). 5% milk was used as blocking reagent. antibody dilution was 1:1000 and incubation with primary antibody was overnight at 4C. Multiple band between 75 and 50 kDa were detected. for IF, PFA was used for fixing the cells and 0.5% triton was used for permeabilization. antibody was diluted 1:200 and samples were incubated with primary ab for 1h at RT. a cytoplasmic signal for Carm1 was detected.
 |
FH Kyosuke (Verified Customer) (06-12-2019) | It works well on HUVECs for Western blot.
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