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Cyclin E1 Antibody

Cyclin E1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 11554-1-AP

Synonyms

CCNE, CCNE1, cyclin E1, G1/S specific cyclin E1



Tested Applications

Positive WB detected inHT-29 cells, mouse heart tissue, MCF-7 cells, NIH/3T3 cells, HeLa cells, Jurkat cells, human lung tissue, K-562 cells, HepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

11554-1-AP targets Cyclin E1 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity Artemia, human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin E1 fusion protein Ag2110
Full Name cyclin E1
Calculated molecular weight 410 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC035498
Gene symbol CCNE1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis.

Protocols

Product Specific Protocols
WB protocol for Cyclin E1 antibody 11554-1-APDownload protocol
FC protocol for Cyclin E1 antibody 11554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation.

Authors - Ruhui Tian
humanWB

Eur J Med Chem

Design, synthesis and biological evaluation of novel uracil derivatives bearing 1, 2, 3-triazole moiety as thymidylate synthase (TS) inhibitors and as potential antitumor drugs.

Authors - Guo-Qing Lu
humanWB,IHC

Biochem Biophys Res Commun

MicroRNA-107 induces cell cycle arrests by directly targeting cyclin E1 in ovarian cancer.

Authors - Zhenghui Tang
  • KD Validated
humanWB

BMC Cancer

Short-term stimulation with histone deacetylase inhibitor trichostatin a induces epithelial-mesenchymal transition in nasopharyngeal carcinoma cells without increasing cell invasion ability.

Authors - Zhihua Shen
mouseWB

J Cell Mol Med

miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells.

Authors - Baoling Ju
humanWB

J Exp Clin Cancer Res

GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner.

Authors - Fen Lin