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CD36 Polyclonal antibody

CD36 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, hamster and More (2)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 18836-1-AP

Synonyms

CD36, CHDS7, FAT, Fatty acid translocase, Glycoprotein IIIb, GP3B, GP4, GPIIIB, GPIV, PAS 4, PAS IV, PASIV, Platelet collagen receptor, Platelet glycoprotein 4, Platelet glycoprotein IV, SCARB3, Thrombospondin receptor



Tested Applications

Positive WB detected inHuman peripheral blood platelets, mouse spleen tissue, THP-1 cells, mouse liver tissue, HepG2 cells, RAW 264.7 cells
Positive IHC detected inhuman spleen tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1000
Sample-dependent, check data in validation data gallery

Product Information

18836-1-AP targets CD36 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, hamster samples.

Tested Reactivity human, mouse, hamster
Cited Reactivity human, mouse, piglet, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name CD36 molecule (thrombospondin receptor)
Calculated molecular weight 472 aa, 53 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC008406
Gene symbol CD36
Gene ID (NCBI) 948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease
  • Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

  • Polymorphisms in CD36 can increase susceptibility to malaria.

  • AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

  • Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer’s disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.            

Protocols

Product Specific Protocols
WB protocol for CD36 antibody 18836-1-APDownload protocol
IHC protocol for CD36 antibody 18836-1-APDownload protocol
FC protocol for CD36 antibody 18836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human ,mouseWB,IF

Oncogenesis

CD36 plays a critical role in proliferation, migration and tamoxifen-inhibited growth of ER-positive breast cancer cells.

Authors - Yu Liang
humanWB

J Obstet Gynaecol Res

Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.

Authors - Qi Xie
mouseWB

Biochem Biophys Res Commun

ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.

Authors - Shujian Wei
pigletWB

J Anim Sci

Antimicrobial peptide KR-32 alleviates Escherichia coli K88-induced fatty acid malabsorption by improving expression of FATP4.

Authors - H Liu
mouseWB

Physiol Rep

Adropin reduces blood glucose levels in mice by limiting hepatic glucose production.

Authors - Dharendra Thapa
humanWB

Sci Rep

An in vitro model of foam cell formation induced by a stretchable microfluidic device.

Authors - Xiaoyang Gu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maurice (Verified Customer) (10-24-2019)

Very clear signals, no undetermined bands like others antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Breast cancer cell lines and breast differentiated adipocytes