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Tested Applications
| Positive IHC detected in | human appendicitis tissue, human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1600-1:6400 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
60209-1-Ig targets CD7 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD7 fusion protein Ag1805 Predict reactive species |
| Full Name | CD7 molecule |
| Calculated Molecular Weight | 240 aa, 25 kDa |
| GenBank Accession Number | BC009293 |
| Gene Symbol | CD7 |
| Gene ID (NCBI) | 924 |
| ENSEMBL Gene ID | ENSG00000173762 |
| RRID | AB_10953398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09564 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD7 antibody 60209-1-Ig | Download protocol |
| IHC protocol for CD7 antibody 60209-1-Ig | Download protocol |
| IF protocol for CD7 antibody 60209-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain | ||
Small In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism |
