CD7 Monoclonal antibody

CD7 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G10D8

Cat no : 60209-1-Ig

Synonyms

CD7, CD7 molecule, GP40, LEU 9, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, Tp40, TP41

Validation Data Gallery

  • IHC staining of human appendicitis using 60209-1-Ig

    IHC staining of human appendicitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 60209-1-Ig

    IHC staining of human appendicitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60209-1-Ig

    IF Staining of human tonsillitis using 60209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60209-1-Ig

    IF Staining of human tonsillitis using 60209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman appendicitis tissue, human tonsillitis tissue, mouse thymus tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1600-1:6400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60209-1-Ig is CD7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD7 fusion protein Ag1805
Full Name CD7 molecule
Calculated molecular weight 240 aa, 25 kDa
GenBank accession numberBC009293
Gene symbol CD7
Gene ID (NCBI) 924
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocols

Product Specific Protocols
WB protocol for CD7 antibody 60209-1-IgDownload protocol
IHC protocol for CD7 antibody 60209-1-IgDownload protocol
IF protocol for CD7 antibody 60209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols