CD74 Monoclonal antibody
CD74 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2D8A1
Cat no : 66390-1-Ig
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Raji cells, Ramos cells, Daudi cells |
| Positive IF detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 2 publications below |
Product Information
66390-1-Ig targets CD74 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD74 fusion protein Ag25503 |
| Full Name | CD74 molecule, major histocompatibility complex, class II invariant chain |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC018726 |
| Gene Symbol | CD74 |
| Gene ID (NCBI) | 972 |
| RRID | AB_2881766 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD74 antibody 66390-1-Ig | Download protocol |
| IF protocol for CD74 antibody 66390-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunol Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1. | ||
Neural Regen Res Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect | ||
Nat Commun Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment | ||
Am J Physiol Cell Physiol WISP1 induces the expression of macrophage migration inhibitory factor (MIF) in human lung fibroblasts through Src kinases and EGFR-activated signaling pathways |
