CoraLite® Plus 488-conjugated CD74 Monoclonal antibody

CD74 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D8A1

Cat no : CL488-66390

Synonyms

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33

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Validation Data Gallery

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  FC experiment of Raji using CL488-66390

  1X10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD74 (CL488-66390, Clone:2D8A1) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: Raji cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-66390 targets CD74 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD74 fusion protein Ag25503
• Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 31 kDa
• GenBank accession number: BC018726
• Gene symbol: CD74
• Gene ID (NCBI): 972
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C