CDK2 Antibody

CDK2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4E2E10

Cat no : 60312-1-Ig

Synonyms

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)



Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60312-1-Ig targets CDK2 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CDK2 fusion protein Ag21310
Full Name cyclin-dependent kinase 2
Calculated molecular weight 33 kDa
Observed molecular weight 37 kDa
GenBank accession number BC003065
Gene symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 60312-1-IgDownload protocol
IHC protocol for CDK2 antibody 60312-1-IgDownload protocol
FC protocol for CDK2 antibody 60312-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Ophthalmol

LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

Authors - Yong Wang
humanWB,IHC

FEBS Open Bio

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

Authors - Changyu Chen
mouseWB

Immunol Invest

Bovine Milk Exosomes Affect Proliferation and Protect Macrophages against Cisplatin-Induced Cytotoxicity.

Authors - Svjetlana Matic
humanWB

Toxicol Lett

The role of the ATM/Chk/P53 pathway in mediating DNA damage in hand-foot syndrome induced by PLD.

Authors - Jie Yang
humanWB

Oncotarget

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Authors - Delong Meng