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- KD/KO Validated
CDK4 Polyclonal antibody
CDK4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11026-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, rat pancreas tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11026-1-AP targets CDK4 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDK4 fusion protein Ag1505|
|Full Name||cyclin-dependent kinase 4|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC010153|
|Gene ID (NCBI)||1019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).
|Product Specific Protocols|
|WB protocol for CDK4 antibody 11026-1-AP||Download protocol|
|IHC protocol for CDK4 antibody 11026-1-AP||Download protocol|
|IF protocol for CDK4 antibody 11026-1-AP||Download protocol|
|IP protocol for CDK4 antibody 11026-1-AP||Download protocol|
|FC protocol for CDK4 antibody 11026-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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