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CEACAM3-Specific Polyclonal antibody

CEACAM3-Specific Polyclonal Antibody for FC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19496-1-AP

Synonyms

Carcinoembryonic antigen CGM1, CD66D, CEACAM3, CGM1, W264, W282

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEACAM3-Specific Polyclonal antibody (19496-1-AP)

    IHC staining of human colon cancer using 19496-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CEACAM3-Specific Polyclonal antibody (19496-1-AP)

    IHC staining of human colon cancer using 19496-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CEACAM3-Specific Polyclonal antibody (19496-1-AP)

    IHC staining of human stomach cancer using 19496-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CEACAM3-Specific Polyclonal antibody (19496-1-AP)

    IHC staining of human stomach cancer using 19496-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of A431 cells using CEACAM3-Specific Polyclonal antibody (19496-1-AP)

    FC experiment of A431 using 19496-1-AP

    1X10^6 A431 cells were stained with .2ug CEACAM3-Specific antibody (19496-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19496-1-AP targets CEACAM3-Specific in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name carcinoembryonic antigen-related cell adhesion molecule 3
Calculated Molecular Weight 27 kDa
GenBank Accession NumberNM_001815
Gene Symbol CEACAM3
Gene ID (NCBI) 1084
RRIDAB_2878582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEACAM3, also named as CD66D and CGM1, belongs to the immunoglobulin superfamily and CEA family. CEACAM3 is the major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species. CEACAM3 plays an important role in the clearance of pathogens by the innate immune system. It is responsible for RAC1 stimulation in the course of pathogen phagocytosis. The antibody is specific to isoform1 of CEACAM3.

Protocols

Product Specific Protocols
IHC protocol for CEACAM3-Specific antibody 19496-1-APDownload protocol
FC protocol for CEACAM3-Specific antibody 19496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Cell Biosci

Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.

Authors - Jiaqi Jin
View Article