|Positive WB detected in||mouse testis tissue|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11517-1-AP targets CENPJ in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CENPJ fusion protein Ag2078|
|Full Name||centromere protein J|
|Calculated molecular weight||1338 aa, 153 kDa|
|Observed molecular weight||153 kDa|
|GenBank accession number||BC024209|
|Gene ID (NCBI)||55835|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.
Cenpj regulates cilia disassembly and neurogenesis in the developing mouse cortex.
Mol Biol Cell
CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells.
Biochim Biophys Acta
Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation.
Albatross/FBF1 contributes to both centriole duplication and centrosome separation.
Stabilization of Cartwheel-less Centrioles for Duplication Requires CEP295-Mediated Centriole-to-Centrosome Conversion.
Mol Biol Cell
NEK7 is required for G1 progression and procentriole formation.