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- KD/KO Validated
CENPJ Polyclonal antibody
CENPJ Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 11517-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 14 publications below |
| IF | See 30 publications below |
Product Information
11517-1-AP targets CENPJ in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebra fish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CENPJ fusion protein Ag2078 |
| Full Name | centromere protein J |
| Calculated molecular weight | 1338 aa, 153 kDa |
| Observed molecular weight | 153 kDa |
| GenBank accession number | BC024209 |
| Gene symbol | CENPJ |
| Gene ID (NCBI) | 55835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPJ antibody 11517-1-AP | Download protocol |
| IF protocol for CENPJ antibody 11517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Cenpj regulates cilia disassembly and neurogenesis in the developing mouse cortex.
| ||
Mol Biol Cell CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells. | ||
Biochim Biophys Acta Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation. | ||
Genes Cells Albatross/FBF1 contributes to both centriole duplication and centrosome separation. | ||
Cell Rep Stabilization of Cartwheel-less Centrioles for Duplication Requires CEP295-Mediated Centriole-to-Centrosome Conversion. | ||
