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  • KD/KO Validated

CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11517-1-AP

Synonyms

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAG 3 associated protein, LAP, LIP1, LYST interacting protein 1, MCPH6



Tested Applications

Positive WB detected inmouse testis tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11517-1-AP targets CENPJ in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebra fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPJ fusion protein Ag2078
Full Name centromere protein J
Calculated molecular weight 1338 aa, 153 kDa
Observed molecular weight 153 kDa
GenBank accession numberBC024209
Gene symbol CENPJ
Gene ID (NCBI) 55835
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

Protocols

Product Specific Protocols
WB protocol for CENPJ antibody 11517-1-APDownload protocol
IF protocol for CENPJ antibody 11517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

J Neurosci

Cenpj regulates cilia disassembly and neurogenesis in the developing mouse cortex.

Authors - Wenyu Ding
  • KO Validated
humanIF

Mol Biol Cell

CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells.

Authors - Divya Ganapathi Sankaran
zebra fishWB

Biochim Biophys Acta

Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation.

Authors - Ji Hyun Kim
mouseIF

Genes Cells

Albatross/FBF1 contributes to both centriole duplication and centrosome separation.

Authors - Akihito Inoko
humanIF

Cell Rep

Stabilization of Cartwheel-less Centrioles for Duplication Requires CEP295-Mediated Centriole-to-Centrosome Conversion.

Authors - Denisse Izquierdo
humanWB,IF

Mol Biol Cell

NEK7 is required for G1 progression and procentriole formation.

Authors - Akshari Gupta