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CEP152 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 21815-1-AP

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Con: 43 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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CEP152 Antibody IHC human brain tissue 21815-1-AP

Immunohistochemistry of paraffin-embedded human brain tissue slide using 21815-1-AP( CEP152 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CEP152 Antibody WB mouse brain tissue 21815-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 21815-1-AP( CEP152 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

CEP152 Antibody IP mouse brain tissue 21815-1-AP

IP Result of anti-CEP152 (IP:21815-1-AP, 3ug; Detection:21815-1-AP 1:500) with mouse brain tissue lysate 9500ug.

CEP152 Antibody IHC human brain tissue 21815-1-AP

Immunohistochemistry of paraffin-embedded human brain tissue slide using 21815-1-AP( CEP152 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse
Positive WB detected in:
mouse brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human brain tissue
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
centrosomal protein 152kDa
Calculated molecular weight:
189 kDa
Observed molecular weight:
110 kDa
GenBank accession number:
NM_014985
Gene ID (NCBI):
22995
Gene symbol
CEP152
Synonyms
centrosomal protein 152kDa, Centrosomal protein of 152 kDa, CEP152, KIAA0912

Background

CEP152, also named as KIAA0912, is a regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. It is necessary for centrosome duplication. CEP152 functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation. CEP152, ATR and CENPJ control cellular responses to DNA damage. CEP152 has some isoforms woth MW 189 kDa, 152 kDa, 110 kDa and 66 kDa.


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